In vitro assessment of probiotic potentials of yeast strains isolated from a cereal-based (Ogi)) traditional fermented food
1 Department of Applied Microbiology, Faculty of Biosciences, Nnamdi Azikiwe University, Awka, Nigeria.
2 Department of Microbiology, Faculty of Biological Sciences, University of Nigeria Nsukka, Nsukka, Nigeria.
Research Article
Open Access Research Journal of Science and Technology, 2024, 11(02), 106–115.
Article DOI: 10.53022/oarjst.2024.11.2.0100
Publication history:
Received on 14 June 2024; revised on 28 July 2024; accepted on 30 July 2024
Abstract:
This present research focused on the isolation of yeast strains from fermented African cereal-based food with promising probiotic attributes. Yeast isolates were obtained from a locally produced cereal-based food “Ogi” also known as Akamu. The yeast was evaluated for their stress tolerance (pH, bile, temperature, NaCl, gastric juice), adhesion properties (auto-aggregation, hydrophobicity) and susceptibility to antibiotics. The strains performed well at 37 °C and exhibited strong ability to tolerate acid (pH 3) and bile salt (1.5%) environment with a percentage survival range of 60-82.61% and 62.96-83.67% respectively. Candida tropicalis (A4) gave the highest salt tolerance at 1% (89.29) and 4% (92.86), while Saccharomyces cerevisiae (A2) showed greater ability to survive the stimulated gastrointestinal conditions at 42.86%. The strains exhibited high auto-aggregation (68.8-94.9%) and a good hydrophobicity to chloroform and toluene. Susceptibility profile of the yeast strains to antibacterial antibiotics showed that all the yeasts presented a resistance pattern. These results indicate that C. tropicalis and S. cerevisiae both have the capability to serve as probiotic agents and can be proposed for various applications. Also, fermented cereal-based Ogi with its wide array of health benefits can serve as a carrier for the delivery of probiotics to both animals and humans as food supplements.
Keywords:
Probiotics; Yeast; Health attributes; Ogi; Adhesion; Antibiotics
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